The company expects annual cost synergies of about $75 million within three years of the close of the transaction, and about $40 million in the first year.
Hologic, which makes cervical cancer tests and mammography systems, expects the deal to close in the second half of the year.
Hologic, which has more than 500 employees in China, said it plans to market Gen-Probe's products including blood tests and sexually transmitted disease tests in both developed and emerging markets.
Goldman Sachs & Co and Perella Weinberg Partners are acting as financial advisers to Hologic, while Morgan Stanley & Co is advising Gen-Probe.
Gen-Probe also reported better-than-expected first-quarter results, helped by strong sales of its women's health products.
Separately, Conceptus Inc said it settled a patent infringement litigation related to Hologic's Adiana Permanent Contraception System.
Under the settlement, Hologic agreed to remove its Adiana system from the market by May 18.
Shares of Hologic closed at $21.23 on Friday on the Nasdaq, while those of Gen-Probe were up about 20 percent at $82.25 before the bell on Monday.
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